Journal article
A. Naik, S. Pernal, Kenneth T. Lewis, Yaobin Wu, Hongkai Wu, Nicholas J. Carruthers, P. Stemmer, B. Jena
The FASEB Journal, 2019
The FASEB Journal, 2019
Semantic Scholar
DOI
Cite
Cite
APA
Click to copy
Naik, A., Pernal, S., Lewis, K. T., Wu, Y., Wu, H., Carruthers, N. J., … Jena, B. (2019). Human Skeletal Muscle‐on‐a‐Chip. The FASEB Journal.
Chicago/Turabian
Click to copy
Naik, A., S. Pernal, Kenneth T. Lewis, Yaobin Wu, Hongkai Wu, Nicholas J. Carruthers, P. Stemmer, and B. Jena. “Human Skeletal Muscle‐on‐a‐Chip.” The FASEB Journal (2019).
MLA
Click to copy
Naik, A., et al. “Human Skeletal Muscle‐on‐a‐Chip.” The FASEB Journal, 2019.
BibTeX Click to copy
@article{a2019a,
title = {Human Skeletal Muscle‐on‐a‐Chip},
year = {2019},
journal = {The FASEB Journal},
author = {Naik, A. and Pernal, S. and Lewis, Kenneth T. and Wu, Yaobin and Wu, Hongkai and Carruthers, Nicholas J. and Stemmer, P. and Jena, B.}
}
Abstract
A comprehensive understanding of the emergent properties of in vitro skeletal muscle growth and development is still in its infancy. To address this gap in our current knowledge, we have developed a stretchable micropatterned 3D human skeletal muscle platform that recapitulates organized and parallel growth of muscle cells and fibers as opposed to the randomly oriented cells growth on a 2D glass surface. Mass spectrometry of the muscle cells growing on the 3D platform express key myogenic proteins for myoblast fusion required in the formation of muscle tissue, and proteins involved in mitochondrial health and biogenesis, in contrast to cells growing on 2D glass surface. These results demonstrate that the engineered human muscle cells grown on the 3D platform holds great promise to further establish the emergent properties of in vitro skeletal muscle growth and development for a wide range of biomedical applications such as transplants, drug screening and gene therapy.